Abstract
Introduction
Breast cancer (BCa) remains the most common cancer in women worldwide. It has been
shown that microRNAs (miRs) play essential roles in tumorigenesis and progression
in many types of cancers, including BCa. We assessed the role of miR-766 on the proliferation,
chemosensitivity, migration, and invasion of BCa cells.
Materials and Methods
The effect of miR-766 on the proliferation of MCF-7 and T47D BCa cells was evaluated
using the MTT assay. The function of miR-766 on the migration and invasion of MCF-7
and T47D cells was examined using Transwell migration and Matrigel invasion assays.
Protein expression was evaluated by Western blot. The role of miR-766 on 5-fluorouracil–induced
apoptosis in MCF-7 and T47D cells was determined using the Caspase-Glo3/7 assay. A
subcutaneous tumor xenograft was performed to examine the effect of miR-766 on tumor
growth in vivo.
Results
Upregulation of miR-766 improved the proliferation, invasion, and migration of BCa
cells. Furthermore, miR-766 reduced the sensitivity of MCF-7 and T47D cells to 5-fluorouracil
treatment. The tumor xenograft experiment showed that miR-766 promoted BCa growth
in vivo. miR-766 decreased 5-flurouracil–induced apoptosis by regulation of BAX and
Bcl-2 expression. miR-766 also affected the epithelial–mesenchymal transition by altering
E-cadherin, N-cadherin, SNAIL, and vimentin expression in MCF-7 and T47D cells. Further
study showed that the expression of phosphatase and tensin homolog and phosphorylated
AKT in MCF-7 and T47D cells had changed after aberrant expression of miR-766.
Conclusion: miR-766 displayed important roles in tumorigenesis and progression in
BCa cells and might act as a potential biomarker to predict the chemotherapy response
and progression in BCa.
Keywords
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Article info
Publication history
Published online: September 18, 2020
Accepted:
October 17,
2019
Received in revised form:
October 17,
2019
Received:
March 9,
2019
Identification
Copyright
© 2020 Elsevier Inc. All rights reserved.
