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Downregulation of PDGF-D Inhibits Proliferation and Invasion in Breast Cancer MDA-MB-231 Cells

  • Author Footnotes
    † These authors contributed equally to the study.
    Jing-Feng Lu
    Footnotes
    † These authors contributed equally to the study.
    Affiliations
    Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
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  • Author Footnotes
    † These authors contributed equally to the study.
    Zhi-Qiu Hu
    Footnotes
    † These authors contributed equally to the study.
    Affiliations
    Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
    Search for articles by this author
  • Author Footnotes
    † These authors contributed equally to the study.
    Meng-Xuan Yang
    Footnotes
    † These authors contributed equally to the study.
    Affiliations
    Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
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  • Wei-Yan Liu
    Affiliations
    Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
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  • Gao-Feng Pan
    Affiliations
    Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
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  • Jun-Bin Ding
    Affiliations
    Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
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  • Jia-Zhe Liu
    Affiliations
    Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
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  • Lang Tang
    Affiliations
    Department of Ultrasonography Department, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
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  • Bin Hu
    Correspondence
    Address for correspondence: Bin Hu, MD, Department of Ultrasonography Department, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University. 170 Xinsong Rd, Shanghai, China
    Affiliations
    Department of Ultrasonography Department, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
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  • Hong-Chang Li
    Correspondence
    Address for correspondence: Hong-chang Li, MD, Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, 170 Xinsong Rd, Shanghai, China. Phone: +86 13816591384.
    Affiliations
    Department of General Surgery, Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai 201100, China
    Search for articles by this author
  • Author Footnotes
    † These authors contributed equally to the study.

      Abstract

      Background

      The platelet derived growth factor-D (PDGF-D) plays an important role in breast tumor aggressiveness. However, limited study has investigated the effect of silencing PDGF-D on the biological function of breast cancer. The purpose of this study is to clarify the potential value of PDGF-D as a target for breast cancer treatment.

      Methods

      Reverse transcription-polymerase chain reaction and western blot were used to detect PDGF-D expression in 5 different breast cancer cells. The lentiviral vector was usd to silence PDGF-D in MDA-MB-231 cells. Then, Methyl Thiazolyl Tetrazolium was used to detect cell viability, 5-Ethynyl-2’- deoxyuridine and a soft agar assay were used to detect cell proliferation and clonality. Additionally, cell apoptosis after PDGF-D knockdown was measured by Annexin V/ Prodium Iodide staining, and cell migration was detected by trans-well assay. Survival rate and tumor size were measured by nude mice transplantation.

      Results

      The MDA-MB-231 and SK-BR-3 cell lines showed higher PDGF-D expression than the MCF7 cell lines (P<.05). After the PDGF-D gene was silenced, the growth and colony forming abilitys ignificantly decreased (P<.05) together with the induction of apoptosis in MDA-MB-231 cells (P<.05). Moreover, MDA-MB-231 cells with PDGF-D silencing showed significantly diminished aggressive migration and invasion potential compared to other cells (P<.05). In vivo experiments also indicated that PDGF-D silencing inhibited tumor growth and improved the survival rate of tumor-bearing mice.

      Conclusion

      Downregulation of PDGF-D had dramatic effects on breast cancer cell proliferation, apoptosis and migration, which indicates that it plays an important role in breast cancer development and progression.

      Keywords

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