Abstract
Background
Increasing studies have shown that microRNAs (miRNAs) have great diagnostic value
in cancer. Axillary lymph node metastasis (ALNM) is closely related to the prognosis
of breast cancer. However, it remains unknown whether miRNAs in whole blood could
be promising biomarkers in breast cancer ALNM.
Methods
An miRNA microarray was used to screen potential differentially expressed miRNA candidates
in whole blood of three breast cancer patients with ALNM and three without ALNM. Quantitative
real-time polymerase chain reaction (qRT-PCR) was used to detect candidate differentially
expressed miRNAs in the whole blood of 109 breast cancer patients. Furthermore, bioinformatics
analysis was carried to predict the potential targets and enriched pathway of miRNAs.
Results
QRT-PCR validated the fact that miR-367-3p, miR-548aq-5p and miR-4710 are downregulated
in breast cancer with ALNM compared to it without ALNM. Receiver operating characteristic
(ROC) curve analysis revealed that miR-367-3p, miR-548aq-5p and miR-4710 have good
diagnostic values. Notably, the three-miRNA signature showed better predictive value,
with an area under ROC curve (AUC) of 0.7414. Bioinformatics analysis revealed that
the miRNAs could participate in a complex network and thus be involved in cancer-related
pathways.
Conclusions
Our findings support the potential of miR-367-3p, miR-548aq-5p and miR-4710 and the
three-miRNA signature as biomarkers for breast cancer with ALNM.
Keywords
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Article info
Publication history
Published online: December 02, 2022
Accepted:
November 29,
2022
Received in revised form:
October 18,
2022
Received:
May 14,
2022
Editor: Dr Y AbdouIdentification
Copyright
© 2022 Elsevier Inc. All rights reserved.
