Research Article| Volume 23, ISSUE 3, P302-316, April 2023

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In vitro Anti-malignant Property of PCMT1 Silencing and Identification of the SNHG16/miR-195/PCMT1 Regulatory Axis in Breast Cancer Cells

  • Author Footnotes
    # Zhongji Zhang and Fengbo Li contributed equally to this study.
    Zhongji Zhang
    # Zhongji Zhang and Fengbo Li contributed equally to this study.
    Department of Thyroid and Breast Surgery, Nanyang First People's Hospital Affiliated to Henan University, Nanyang, China

    Key Laboratory of Thyroid Tumor Prevention and Treatment, Nanyang First People's Hospital Affiliated to Henan University, Nanyang, China
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  • Fengbo Li
    Address for correspondence: Guangwei Jia, MD. Department of Thyroid and Breast Surgery, Nanyang First People's Hospital Affiliated to Henan University, No. 12 Renmin Road, Nanyang 473012, China.
    Department of Respiratory Medicine, Nanshi Hospital of Nanyang, Nanyang, China
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  • Yan Li
    Department of General Surgery, Nanyang First People's Hospital Affiliated to Henan University, Nanyang, China
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  • Zhong Li
    Department of General Surgery, Nanyang First People's Hospital Affiliated to Henan University, Nanyang, China
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  • Guangwei Jia
    Address for correspondence: Guangwei Jia, MD. Department of Thyroid and Breast Surgery, Nanyang First People's Hospital Affiliated to Henan University, No. 12 Renmin Road, Nanyang 473012, China.
    Department of Thyroid and Breast Surgery, Nanyang First People's Hospital Affiliated to Henan University, Nanyang, China
    Search for articles by this author
  • Author Footnotes
    # Zhongji Zhang and Fengbo Li contributed equally to this study.
Published:December 23, 2022DOI:


      • PCMT1 knockdown inhibited proliferation and the invasive ability in breast cancer cells.
      • PCMT1 silencing triggered apoptosis in breast cancer cells.
      • miR-195 was identified as the only upstream miRNA of PCMT1 in breast cancer.
      • SNHG16 was screened out as the only upstream lncRNA of miR-195 in breast cancer.



      Protein L-isoaspartate (D-aspartate) O-methyltransferase (PCMT1) is a highly conserved protein repair enzyme that participates in regulating the progression of human cancers. We therefore studied the function and the related mechanisms of PCMT1 in breast cancer cells.


      Expression profile and prognostic analysis of PCMT1 in breast cancer patients were analyzed using online databases. PCMT1 expression in breast cancer cells was detected by western blot analysis. Cell proliferation was determined by CCK-8 and colony formation assays. Apoptosis was evaluated using flow cytometry analysis and caspase-3/7 activity assay. Cell invasion was assessed by Transwell invasion assay. The small nucleolar RNA host gene 16 (SNHG16)/miR-195/PCMT1 regulatory axis was identified using bioinformatics analysis.


      PCMT1 expression was increased in breast cancer tissues and cells. High PCMT1 expression was correlated with poor prognosis in breast cancer patients. PCMT1 knockdown suppressed cell proliferation and colony formation ability in breast cancer cells. Moreover, PCMT1 knockdown induced apoptosis and restrained the invasive ability in breast cancer cells. PCMT1 overexpression increased the proliferative and invasive abilities of breast cancer cells. miR-195 was identified as the unique upstream miRNA of PCMT1. SNHG16 was identified as the unique upstream lncRNA of miR-195. SNHG16 knockdown downregulated PCMT1 by increasing miR-195 expression. Breast cancer cell proliferation was regulated by the SNHG16/miR-195/PCMT1 axis.


      PCMT1 silencing inhibited cell proliferation and invasion and induced apoptosis in breast cancer cells and the SNHG16/miR-195/PCMT1 regulatory axis might serve as a potential therapeutic target for breast cancer.


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