Highlights
- •Minor allele of rs440960, but not rs1470579, was significantly associated with breast cancer (BC).
- •Significantly higher rs4402960 T/T genotype frequency was noted in BC cases.
- •Lower rs1470579 minor allele and genotype frequencies seen in triple negative BC (TNBC) cases.
- •Both susceptible and protective 2-locus haplotypes associated with TNBC.
Abstract
Background
Several studies documented that insulin-like growth factor-2 mRNA-binding protein
2 (IGF2BP2) contributes to carcinogenesis, and 1 report documented the association
of IGF2BP2 rs4402960 with increased risk of breast cancer (BC). This study investigated the
association of rs4402960 and rs1470579 IGF2BP2 variants with BC and triple negative BC (TNBC).
Materials and Methods
This case-control study included 488 BC patients comprising 130 TNBC and 358 non-TNBC
patients, and 476 cancer-free controls. Genomic DNA was obtained from peripheral venous
blood, and genotyping was done by allelic exclusion method on real-time PCR.
Results
The rs440960, but not rs1470579, minor allele was significantly associated with BC,
and significantly higher rs4402960 T/T genotype frequency was noted in BC patients
than controls; the distribution of rs1470579 genotypes were comparable between BC
patients and controls. In contrast, significantly lower rs1470579 minor allele frequency,
and reduced rs1470579 A/C and C/C, and rs4402960 T/T genotype frequencies were seen
in TNBC cases. Among TNBC cases, rs4402960 and rs1470579 correlated with menses pattern,
histological type, breastfeeding, oral contraceptive use and hormonotherapy. Among
non-TNBC patients, and rs1470579 correlated significantly with breast feeding, oral
contraceptive use, hormonotherapy, and nodal status; rs4402960 also correlated with
menses pattern. Two-locus (rs440960-rs1470579) haplotype analysis confirmed the positive
association of TC, and negative association of GC and TA haplotypes with BC, while
TC and GC haplotypes were negatively associated with TNBC.
Conclusion
Whereas rs440960 was positively associated with BC, both rs4402960 and rs1470579 were
negatively associated with TNBC, suggesting potential diagnostic/prognostic role in
BC and its complications.
Keywords
Abbreviations:
BC (breast cancer), CI (confidence interval), ER (estrogen receptor), HER/2-neu (human epidermal growth factor receptor 2), IGF2 (insulin-like growth factor-2), IGF2BP2 (insulin-like growth factor-2 mRNA-binding protein 2), LN (lymph nodes), OR (odds ratio), PR (progesterone receptor), TNBC (triple negative BC)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: December 30, 2022
Accepted:
December 22,
2022
Received in revised form:
December 20,
2022
Received:
August 1,
2022
Identification
Copyright
© 2022 Elsevier Inc. All rights reserved.
